Human Anti-CLDN18 Recombinant Antibody (clone 25E3)
CAT#: ZG-0140U
Human anti-CLDN18 antibody (clone 25E3) displayed high specificity against the CLDN18 protein of Human.
Tested Data
Gene Expression
FC
Figure 1 Human Anti-CLDN18 Antibody (ZG-0140U) in FC
Untransfected KATO3 cells (green line), transfected Human CLDN18.2 KATO3 stable cells (red line) and transfected Human CLDN18.1 NIH-3T3 stable cells (blue line) were stained with anti-CLDN18 antibody (2µg/1*106 cells), washed and then followed by FITC-conjugated anti-Human IgG Fc antibody (ZG-0140U) and analyzed with flow cytometry.
ELISA
Figure 2 Human Anti-CLDN18 Antibody (ZG-0140U) in ELISA
The Binding Activity of Human CLDN18.2 with Anti-CLDN18 recombinant antibody. Activity: Measured by its binding ability in a functional ELISA. Immobilized Human CLDN18.2 (ZG-0140U) at 5 μg/mL can bind Anti-CLDN18 recombinant antibody, the EC50 is 2.947-8.782 ng/mL.
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Subcellular Location
Figure 1 IF staining of human cell line OE19
Immunofluorescent staining of human cell line OE19 shows localization to cell junctions.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18446/1971_G5_3_selected.jpg
Normal Tissue
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong membranous and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13010/ihc_selected.jpg
Normal Tissue
Figure 3 Stomach
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13243/28279_A_2_1.jpg
RNA Expression
Figure 4 RNA cell line category: Cell line enriched (OE19)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066405-CLDN18
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Specifications
- Immunogen
- Recombinant Human CLDN18 protein
- Host Species
- Human
- Type
- Humanized IgG1
- Specificity
- Human CLDN18
- Species Reactivity
- Human
- Clone
- 25E3
- Applications
- ELISA, FC
- Conjugate
- Unconjugated
- Related Disease
- Bile Duct Cancer and Urachus Cancer
Product Property
- Purification
- Affinity Purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Flow Cytometry. Specific conditions should be optimized by the end user.
FC:1:50-1:200
Target
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Knockout Validation Aid
This antibody perfectly suited our needs for validating knockdown experiments. Its reliability was confirmed by the clear specificity and lack of signal in knockout samples.
Ideal for Routine Lab Use
Incorporating this antibody into our routine lab procedures has been incredibly beneficial. Consistency, reliability, and clear results have streamlined our workflows significantly.
Cost-Efficient
Considering its performance and versatility across multiple applications, this antibody provides excellent value. It's a smart investment for any lab looking for high-quality reagents.
Q&As
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What are "housekeeping" antibodies?
A: Housekeeping antibodies target proteins consistently expressed at stable levels and are used as loading controls in applications like Western blots.
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How does antibody valency affect binding?
A: Antibody bivalency increases binding avidity and stability when interacting with multivalent antigens.
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Can antibodies be used for identifying protein-protein interactions?
A: Co-immunoprecipitation with specific antibodies can help study protein-protein interactions effectively.
View the frequently asked questions answered by Creative Biolabs Support.
Cite This Product
To accurately reference this product in your publication, please use the following citation information:
(Creative Biolabs Cat# ZG-0140U, RRID: AB_3112064)
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
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